BMB Reports 2018; 51(9): 462-467  https://doi.org/10.5483/BMBRep.2018.51.9.153
Potential involvement of Drosophila flightless-1 in carbohydrate metabolism
Jung-Eun Park1,#, Jinho Jang2,#, Eun Ji Lee1, Su Jung Kim3, Hyun Ju Yoo3, Semin Lee2,* & Min-Ji Kang1,*
1Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, 2Department of Biological Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan 44919, 3Department of Convergence Medicine, Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Korea
Correspondence to:

Semin Lee, Tel: +82-52-217-2663; Fax: +82-52-217-2299; E-mail: seminlee@unist.ac.kr; Min-Ji Kang, Tel: +82-2-3010-2797; Fax: +82-2-3010-5307; E-mail: mjkang@amc.seoul.kr
#These authors contributed equally to this work.

Received: July 10, 2018; Revised: July 24, 2018; Accepted: July 25, 2018; Published online: September 30, 2018.
© Korean Society for Biochemistry and Molecular Biology. All rights reserved.

cc This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
A previous study of ours indicated that Drosophila flightless-1 controls lipid metabolism, and that there is an accumulation of triglycerides in flightless-1 (fliI)-mutant flies, where this mutation triggers metabolic stress and an obesity phenotype. Here, with the aim of characterizing the function of FliI in metabolism, we analyzed the levels of gene expression and metabolites in fliI-mutant flies. The levels of enzymes related to glycolysis, lipogenesis, and the pentose phosphate pathway increased in fliI mutants; this result is consistent with the levels of metabolites corresponding to a metabolic pathway. Moreover, high-throughput RNA sequencing revealed that Drosophila FliI regulates the expression of genes related to biological processes such as chromosome organization, carbohydrate metabolism, and immune reactions. These results showed that Drosophila FliI regulates the expression of metabolic genes, and that dysregulation of the transcription controlled by FliI gives rise to metabolic stress and problems in the development and physiology of Drosophila.
Keywords: Flightless-1, Gene expression, Metabolism, Metabolomics, RNA sequencing


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