BMB Reports 2018; 51(7): 338-343  https://doi.org/10.5483/BMBRep.2018.51.7.032
Investigation of functional roles of transcription termination factor-1 (TTF-I) in HIV-1 replication
Seong-Hyun Park1, Kyung-Lee Yu1, Yu-Mi Jung1, Seong-Deok Lee1, Min-Jeong Kim2 & Ji-Chang You1,2,*
1National Research Laboratory for Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea, Seoul 06591, 2Avixgen Inc., Seoul 06649, Korea
Correspondence to: Tel: +82-2-2258-7312; Fax: +82-2-2258-7790; E-mail: jiyou@catholic.ac.kr
Received: February 10, 2018; Revised: February 28, 2018; Accepted: March 17, 2018; Published online: July 31, 2018.
© Korean Society for Biochemistry and Molecular Biology. All rights reserved.

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Abstract
Transcription termination factor-1 (TTF-I) is an RNA polymerase 1-mediated transcription terminator and consisting of a C-terminal DNA-binding domain, central domain, and N-terminal regulatory domain. This protein binds to a so-called 'Sal box' composed of an 11-base pair motif. The interaction of TTF-I with the 'Sal box' is important for many cellular events, including efficient termination of RNA polymerase-1 activity involved in pre-rRNA synthesis and formation of a chromatin loop. To further understand the role of TTF-I in human immunodeficiency virus (HIV)-I virus production, we generated various TTF-I mutant forms. Through a series of studies of the over-expression of TTF-I and its derivatives along with co-transfection with either proviral DNA or HIV-I long terminal repeat (LTR)-driven reporter vectors, we determined that wild-type TTF-I downregulates HIV-I LTR activity and virus production, while the TTF-I Myb-like domain alone upregulated virus production, suggesting that wild-type TTF-I inhibits virus production and trans-activation of the LTR sequence; the Myb-like domain of TTF-I increased virus production and trans-activated LTR activity.
Keywords: c-Myb, Gene Regulation, HIV-1, HIV-1 Long Terminal Repeat, TTF-I, Virus Latency, Virus Replication


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