Inhibitory effects of SOCS1 on pro-inflammatory cytokine production and ROS generation induced by LPS in THP1 human monocytic cells and mouse BMDMs. Flag or Flag-SOCS1 THP1 cells were differentiated by PMA. Cells were then either not treated (NT) or treated with LPS (1 µg/ml) for the indicated duration. ROS levels were determined (A). The culture supernatants were analyzed to measure inflammatory cytokines by ELISA (B). The sh control and shSOCS1-transduced THP1 cells were stimulated with LPS with or without NAC pretreatment, after which ROS (C) and cytokine levels (D) were determined. BMDMs from C57BL/6 mice were transduced with mouse shSOCS1. The SOCS1 levels were analyzed by western blot (E). ROS levels in shSOCS1 cultures were analyzed as % increase over mock (sh) cultures (F). LPS-induced cytokine production was determined by ELISA (G). Results represent means ± SD obtained from three independent experiments performed in triplicate (*P < 0.05; +n.s.).