BMB Reports : eISSN 1976-670X

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Fig. 2. Reduced expression of TOMM20 diminished characteristics of cancer cells and caused mitochondrial dysfunction in CRC cells. (A, B) siTOMM20 transfection resulted in suppression of TOMM20 expression and stunted cell growth compared to the negative control cells. (C) Reduced migration of CRC cells by suppression of TOMM20 expression. (D) Reduction in invasion of siTOMM20 treated cells and corresponding expressions of E-cadherin and Vimentin. (E) Cell cycle analysis revealed the increased proportion of the S phase cells in siTOMM20 transfected cells. (F) BrdU incorporation experiment showed much increased BrdU-negative S phase cells (P1) in siTOMM20 transfected cells. (G) FACS analysis revealed increased apoptosis in siTOMM20 transfected cells. Western blot analysis revealed increased cleavage activities in siTOMM20 transfected cells. (H) FACS analysis using MitoTracker Green and Red staining showed increase in the number of mitochondria with lost membrane potential (P1). (I) Reduced ATP production in siTOMM20 treated cells. (J) MitoTracker Red staining for live cells and confocal microscopy showed mitochondrial fragmentation in siTOMM20-transfected cells. All quantitative results are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.
BMB Reports 2019;52:712~717 https://doi.org/10.5483/BMBRep.2019.52.12.249
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