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Fig. 4. Effect of p90RSK activation on NF-κB transcriptional activity. (A–C) MDA-MB-231 cells were pretreated with 10 μM of FMK for 1 h followed by treatment with 20 μg/ml of Cis-DDP for 1 h. (A) The p65 expression in cells was observed using a laser scanning confocal spectral microscope (Nanoscope systems). Bars indicate 30 μm. (B) Many nuclear p65 NF-κBs were indicated as fold change compared to the control (0) sample. (C) Total protein lysates were subjected to western blotting using anti-phospho or -total p65 antibodies. (D) MDA-MB-231 cells were co-transfected with the pNF-κB-luc and pRL-Renilla reporter construct. At 18 h after transfection, cells were treated with 10 μM of FMK for 1 h followed by treatment with Cis-DDP or TNF-α (positive control) for 12 h. The luciferase activities of the extracts were determined and normalized based on Renilla luciferase expression. The data are presented as means ± SEM (n = 3). ***P < 0.001 compared with 0 sample; ##P < 0.01 compared with each control.
BMB Reports 2019;52:706~711 https://doi.org/10.5483/BMBRep.2019.52.12.234
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