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Fig. 3. Involvement of p90RSK activation in Cis-DDP-induced EMT. (A–F) MDA-MB-231 cells were pretreated with 10 μM of FMK for 1 h followed by treatment with 20 μg/ml of Cis-DDP for 6 h. (G–L) Cells were transfected with WT-RSK1 or DN-RSK1 plasmids for 18 h followed by treatment with 20 μg/ml of Cis-DDP for 6 h. Total RNA samples were subjected to qPCR against E-cadherin (A, G), N-cadherin (B, H), vimentin (C, I), Snail (D, J), Twist (E, K), and ZEB1 (F, L) primers. The data are presented as means ± SEM (n = 3). *P < 0.05, **P < 0.01 or ***P < 0.001 compared with 0 sample; #P < 0.05, ##P < 0.01 or ###P < 0.001 compared with each control.
BMB Reports 2019;52:706~711 https://doi.org/10.5483/BMBRep.2019.52.12.234
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