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Fig. 1. Inhibition of p90RSK decreases cell proliferation in MDAMB-231 cells. (A) MDA-MB-231, BT549, and MCF-7 breast cancer cells were stimulated with Cis-DDP for 24 h and cell viability was determined by an MTT assay. (B) Cells were treated with the Cis-DDP for 36 h and cell proliferation was determined by FACS using a Ki-67 proliferation kit. (C) MDA-MB-231 cells were stimulated with 20 μg/ml of Cis-DDP or 5 μg/ml of Dox for 24 h and the cell cycle was investigated using FACS. (D) MDA-MB-231 cells were stimulated with Cis-DDP for 24 h and the protein and mRNA expression of p90RSK were determined by western blotting (upper panel) and qPCR (lower bar graph). (E) MDA-MB-231 cells were stimulated with Cis-DDP for 5 min and whole-cell lysates were subjected to western blot analysis against the indicated antibodies. (F, G) MDA-MB-231 cells were treated with 10 μM of FMK for 3 h (F) or transfected with pcDNA or DN-RSK1 for 18 h (G) followed by treatment with the indicated dose of Cis-DDP for 24 h and cell viability was determined by an MTT assay. The data are presented as means ± SEM (n = 3). *P < 0.05, **P < 0.01 or ***P < 0.001 compared with 0 sample; #P < 0.05, ##P < 0.01 or ###P < 0.001 compared with each control.
BMB Reports 2019;52:706~711 https://doi.org/10.5483/BMBRep.2019.52.12.234
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