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Fig. 4.

ACOX1 destabilizes p73 to reduce apoptosis. (A) shACOX1 expressed Raji cells (sh) and control cells (NC) were subjected to WB analysis. (B, C) shACOX1 expressed Raji cells (sh) and control cells (NC) were transfected with p73 siRNA for 48 hours. Cells were subjected to WB analysis (B) or apoptosis analysis (C). (D) ACOX1 expressed Raji cells and control cells (Con.) were treated with doxorubicin (0.6 μM) or control solvent for 48 hours. Cells were subjected to WB analysis. (E) shACOX1 expressed Raji cells (sh) and control cells (NC) were subjected to RNA extraction and PCR analysis. (F) Cells were treated with MG132 (10 μM). Cells were subjected to Western blot analysis. (G, H) ACOX1 expressed, shACOX1 expressed Raji cells and control cells were treated with CHX for the indicated time. WB analysis of p73, ACOX1, and GAPDH was performed (G). The densitometric quantification of p73 normalized to GAPDH was plotted against various time points to determine its half-life (H).

BMB Reports 2019;52:566~571 https://doi.org/10.5483/BMBRep.2019.52.9.094
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