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Fig. 3.

Downregulation of ACOX1 induced apoptosis through the intrinsic pathway. (A) shACOX1 expressed Raji cells (sh) and control cells (NC) were treated with doxorubicin (0.1 μM) or control solvent for 48 hours. Cells were subjected to WB analysis. (B) shACOX1 expressed Raji cells (sh) and control cells (NC) were treated with LEHD, DEVD, IETD or control solvent for 24 hours. Cells were subjected to apoptosis analysis. Statistical results are shown. (C) shACOX1 expressed Raji cells (sh) and control cells (NC) were subjected to total protein extraction or cytoplasmic/mitochondria protein extraction. These proteins were subjected to WB analysis. (D) Treatment was similar with (A). Cells were subjected to mitochondrial membrane potential analysis. (E) ACOX1 expressed Raji cells and control cells (Con.) were treated with doxorubicin (0.6 μM) or control solvent for 48 hours. Cells were subjected to WB analysis. (F) The treatment was similar with (E). Cells were subjected to mitochondrial membrane potential analysis. The bar represents mean ± SD of three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001, The ANOVA test, followed by Least Significant Difference test, were used to make statistical comparisons).

BMB Reports 2019;52:566~571 https://doi.org/10.5483/BMBRep.2019.52.9.094
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