BMB Reports : eISSN 1976-670X

Download original image
Fig. 1.

ACOX1 regulates proliferation and apoptosis. (A) ACOX1 was stably expressed in Raji lymphoma cells via lentivirus-mediated gene transfer. Cells were subjected to WB analysis. (B) Cells (1 × 105) stably expressed ACOX1 were culture for an indicated time. Then MTT assay was used to evaluate proliferation. (C) ACOX1 shRNA was stably expressed in Raji lymphoma cells via lentivirus-mediated gene transfer. Cells were subjected to WB analysis. (B) Cells (1 × 105) stably expressed ACOX1 shRNA were culture for an indicated time. Then MTT assay was used to evaluate proliferation. (E, F) Cells (1 × 106) stably expressed ACOX1 were subjected to apoptosis analysis (E). Statistical results are shown (F). (G, H) Cells (1 × 106) stably expressed ACOX1 were subjected to apoptosis analysis (G). Statistical results are shown (H). (I, J) Treatment was the same as (E) and (F). Cells were subjected to TUNEL assay analysis (I). Statistical results are shown (J). (K, L) Treatment was the same as (G) and (H). Cells were subjected to TUNEL assay analysis (K). Statistical results are shown (L). The bar represents mean ± SD of three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001, The ANOVA test, followed by Least Significant Difference test, were used to make statistical comparisons).

BMB Reports 2019;52:566~571 https://doi.org/10.5483/BMBRep.2019.52.9.094
© BMB Rep.