BMB Reports : eISSN 1976-670X

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Fig. 4. PTEN is a direct target of miR-371. (A) Heat map representing the mRNA expression profile of a panel of cancer-related genes regulated by miR-371 in Hep3B and HepG2 cell lines as determined using real-time PCR. (B) Luciferase reporter activities driven by the 3′UTRs of potential miR-371 targets were examined in Hep3B cells transfected with miR-371 mimics or negative control (NC). (C) Schematic diagram of miR-371 binding site on the 3′UTR of PTEN mRNA. (D) Luciferase reporter activities driven by wild-type or mutant PTEN 3′UTRs (TTTGAG > CAGCTA) were examined in Hep3B cells transfected with miR-371 mimics or negative control (NC). (E) Immunoblot analysis of PTEN protein level in Hep3B and HepG2 cells transfected with miR-371 mimics. Data are shown as mean ± S.D. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant (D: one-way ANOVA; others: Student’s t-test).
BMB Reports 2019;52:312~317 https://doi.org/10.5483/BMBRep.2019.52.5.155
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