BMB Reports : eISSN 1976-670X

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Fig. 1. Step-wise protocol elucidating the endocrine differentiation of hESCs and the characterization of hESC-ECCs. (A) Experimental protocol outlining the stages, and inducing factors and chemicals for differentiation of hESCs into definitive endoderm (DE), pancreatic endoderm (PE), and endocrine progenitor (EP) cells and the final insulin-producing endocrine cells (EC). (B) Phase-contrast image of hESC-ECCs. Scale bar = 100 μm. (C) Immmunocytochemical analysis showing the expression of β cell-associated genes, endocrine hormone genes, and β cell function-linked proteins. Scale bar = 50 μm. The figure is reproduced from the article by Kim et al. 2016 (). This work is licensed under a Creative Commons Attribution 4.0 International License ().
BMB Reports 2019;52:295~303
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