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Fig. 3. Involvement of ROS in Prx I-induced cellular senescence. (A) Expression level of Prx I was examined with immunocytochemistry (ICC) in MEFs (upper panel). Images were counterstained with DAPI. ROS production was detected by the fluorescent CM-H2DCFDA using fluorescence microscopy (lower panel). (B) Intracellular ROS levels were analyzed by flow cytometry (upper panel). Data represent Mean ± SD (n = 3). *P < 0.05. (lower panel). (C) The percentage of cells positively stained for the SA-β-gal activity in MEFs were pre-treated with presence or absence of either 5 mM NAC or 20 μM H2O2 at DIV 3 and 5. SA-β-gal positive cells were counted in at least 10 fields from triplicate plates. Data represent Mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.
BMB Reports 2017;50:528~533
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