BMB Reports : eISSN 1976-670X

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Fig. 1. Prx I−/− MEFs show persistent growth arrest and induce senescence-associated phenotypic changes. (A) MEFs derived from different Prx I genotypes were generated and maintained by a defined 3T3 protocol. (B) Prx I+/+ or Prx I−/− MEFs images were captured at 4 passage under a differential interference contrast (DIC) microscopy. (C) Cell sizes of the indicated MEFs were assessed by measuring forward light scatter (FSC) via flow cytometry. 4P, 4 passage; 6P, 6 passage. Data represent Mean ± SD (n = 3). *P < 0.05. (D) The population doubling level was calculated for Prx I+/+ or Prx I−/− MEFs at each passage every 2 days (upper panel) and cells were counted at 7 days in vitro (DIV) (lower panel). Data represent Mean ± SD (n = 3). *P < 0.05, **P < 0.01.
BMB Reports 2017;50:528~533
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