BMB Reports : eISSN 1976-670X

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Fig. 4. Actin damage induced apoptotic death by ectopic expression of Skp2. (A) Flow cytometric analysis of HCT116 cells and its transfectant expressing flag-tagged Skp2 after treatment with PTX2 for 72 h. Cells were labeled with propidium iodide (PI) as described in Materials and Methods. Data representative of three independent experiments is shown. (B) Apoptotic body formation in PTX2-treated cells. HCT116 cells and its transfectant expressing Skp2 were treated with PTX2 for 72 h and apoptotic body formation (arrows) was determined by DAPI staining then photographing cells under fluorescence microscopy (×200). (C) The roles of Skp2 and p53 in actin inhibitor-induced tetraploid G1 arrest. Actin inhibitors that block cytokinesis lead to Skp2 repression as well as p53 activation. While p53 induces p21 expression, Skp2 repression induces p27 expression. Therefore, actin damage induced tetraploid G1 arrested cells have increased amounts of CDK inhibitors, p21 and p27, which act to inhibit uncoupled DNA replication and nuclear division without cytokinesis.
BMB Reports 2017;50:379~383
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