BMB Reports : eISSN 1976-670X

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Fig. 3. Effects of Skp2 ectopic expression on tetraploid G1 arrest. (A) BrdU incorporation assay. Parental HCT116 cells and Skp2-exressing cells were treated with PTX-2 for the indicated periods and were then subjected to BrdU incorporation assay as described in Materials and Methods. (B) HCT116 and Skp2-expressing cells were treated with PTX-2 for 48 h and then DAPI-stained. The number of nuclei in each cell was counted as described in Materials and Methods. (C) Protein analysis of Skp2 and p27/KIP1. Expression of Flag-Skp2 and p27/kip1 were determined in HCT116 and Skp2-expressing cells treated with PTX2 for 48 h, by Western blotting analysis with the indicated antibodies.
BMB Reports 2017;50:379~383 https://doi.org/10.5483/BMBRep.2017.50.7.063
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