BMB Reports : eISSN 1976-670X

Download original image
Fig. 2. Tet proteins were not essential for vitamin C–induced IL17 expression. (A) FACS-sorted WT and Tet2−/− naïve CD4+ T cells were transfected with control or Tet1/Tet3 siRNAs and cultured under the indicated conditions. IL17 expression was analyzed by flow cytometry. (B) FACS-sorted WT and Tet2−/− naïve CD4+ T cells were transfected with Tet1/Tet3 siRNAs, and the expression of the indicated gene transcripts was checked by RT-PCR. (C) FACS-sorted WT and Tet2−/− naïve CD4+ T cells were transfected with control or Tet1/Tet3 siRNAs and cultured under the indicated conditions. IL17+ cells were re-sorted and subjected to bisulfate sequencing. Results of statistical analysis are shown as the mean ± SEM of the results (n = 3) pooled from two independent experiments. Numbers in the FACS plots of (A) and (C) refer to the percentage of each subset. Methylation status of individual CpG motif was shown by white (demethylation) or black (methylation) circles.
BMB Reports 2017;50:49~54 https://doi.org/10.5483/BMBRep.2017.50.1.193
© BMB Rep.