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Fig. 1. Vitamin C enhances the expression of IL17. (A) FACS-sorted naïve CD4+ T cells were cultured in the presence or absence of vitamin C (10 μg/ml) under Th17 polarizing condition for 3 d and IL17 expression was checked by flow cytometry after PMA/ionomycin restimulation. Data are representative of three independent experiments. (B) FACS-sorted naïve CD4+ T cells were cultured in the presence or absence of vitamin C (10 μg/ml) under Th1 polarizing condition and IFN-γ expression was checked by flow cytometry. Data are representative of two independent experiments. (C) Th17 cells were generated in the presence or absence of vitamin C and then, the expression levels of the indicated gene transcripts were checked by RT-qPCR (mean ± SEM of duplicates, from one experiment representative of three independent experiments). (D) Percentages of IL17 (left) or RORγt (right)–expressing cells cultured under Th17 conditions in the presence of vitamin C ± sulfinpyrazone (sulifin). Bars show the mean ± SEM of the results (n = 6–8) pooled from three independent experiments. FACS plots of (A) and (B) refer to the percentage of each subset. ***P < 0.001; *P < 0.05; NS, not significant.
BMB Reports 2017;50:49~54 https://doi.org/10.5483/BMBRep.2017.50.1.193
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