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Article Info.
2012.11.30; 45(11) pp. 641~646
Title

microRNA-214-mediated UBC9 expression in glioma  

Authors

Zhiqiang Zhao, Xiaochao Tan, Ani Zhao, Liyuan Zhu, Bin Yin, Jiangang Yuan, Boqin Qiang & Xiaozhong Peng*  

Institutions

The National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, PR China  

Abstract

It has been reported that ubiquitin-conjugating enzyme 9 (Ubc9),
the unique enzyme2 in the sumoylation pathway, is up-regulated
in many cancers. However, the expression and regulation of
UBC9 in glioma remains unknown. In this study, we found that
Ubc9 was up-regulated in glioma tissues and cell lines compared
to a normal control. UBC9 knockdown by small interfering RNA
(siRNA) affected cell proliferation and apoptosis in T98G cells.
Further experiments revealed that microRNA (miR)-214 directly
targeted the 3' untranslated region (UTR) of UBC9 and that there
was an inverse relationship between the expression levels of
miR-214 and UBC9 protein in glioma tissues and cells. miR-214
overexpression suppressed the endogenous UBC9 protein and
affected T98G cell proliferation. These findings suggest that
miR-214 reduction facilitates UBC9 expression and is involved
in the regulation of glioma cell proliferation.  

Keywords

Cell proliferation, Glioma, miR-214, Sumoylation, UBC9