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Article Info.
2009.03.31; 42(3) pp. 154~159
Title

The PcG protein hPc2 interacts with the N-terminus of histone demethylase JARID1B and acts as a transcriptional co-repressor  

Authors

Wu Zhou, Haixiang Chen & Lihuang Zhang*  

Institutions

Institute of Immunology, Zhejiang University, Hangzhou 210058, China  

Abstract

JARID1B (jumonji AT rich interactive domain 1B) is a large nuclear protein that is highly expressed in breast cancers and is proposed to function as a repressor of gene expression. In this paper, a phage display screen using the N-terminus of JARID1B as bait identified one of the JARID1B interacting proteins, namely PcG protein (Polycomb group) hPc2. We demonstrated that the C-terminal region, including the COOH box, was required for the interaction with the N-terminus of JARID1B. In a reporter assay system, co-expression of JARID1B with hPc2 significantly enhanced the transcriptional repression. These results support a role for hPc2 acting as a transcriptional co-repressor. [BMB reports 2009; 42(3): 154-159]  

Keywords

Histone demethylase, hPc2, JARID1B, Transcriptional co-repressor